Journal: American Journal of Translational Research

Article Title: Signaling by TGF-betas in tubule cultures of adult rat testis

doi:

Figure Lengend Snippet: Antibodies used for detection of the TBR1-TBR2 signalosome and signaling

Article Snippet: Then the primary antibodies ( ) diluted in DAKO diluent buffer were added and the slides incubated overnight at 4°C. table ft1 table-wrap mode="anchored" t5 caption a7 Protein Provider Cat-No Species Clonality Dilution/concentration TBR1 Santa Cruz Biotechnology (Dallas, USA) sc-33933 Goat polyclonal 1:200 TBR2 Santa Cruz Biotechnology sc-220 Rabbit polyclonal 1:350 rh ALK2 chimera Bio-techne (Wiesbaden, Germany) 637-AR Human rh 5 µM rh ALK3 chimera Bio-techne 315-BR Human rh 5 µM rh ALK6 chimera Bio-techne 505-PR Human rh 5 µM rh IgG1 chimera Bio-techne 110-HG Human rh 5 µM Open in a separate window Cat-No, Catalog-Number; rh, recombinant human.

Techniques:

Journal: American Journal of Translational Research

Article Title: Signaling by TGF-betas in tubule cultures of adult rat testis

doi:

Figure Lengend Snippet: Treatment of tubule cultures with 10 ng/ml TGF-β1 (A) or TGF-β2 (B), respectively, significantly increased phosphorylation of Smad1. (A) The ALK2- and ALK3-specific chimeric inhibitor significantly blocked TGF-β1-induced phosphorylation of Smad1 nearly to control levels (**P<0.01; *P≤0.05). (B) The ALK3- and ALK6-specific chimeric inhibitor significantly blocked TGF-β2-induced phosphorylation of Smad1 nearly to control levels (**P<0.01; *P≤0.05). The control experiment with IgG isotypes did not show any effect. Each experiment was repeated three times in duplicates (A+B).

Article Snippet: Then the primary antibodies ( ) diluted in DAKO diluent buffer were added and the slides incubated overnight at 4°C. table ft1 table-wrap mode="anchored" t5 caption a7 Protein Provider Cat-No Species Clonality Dilution/concentration TBR1 Santa Cruz Biotechnology (Dallas, USA) sc-33933 Goat polyclonal 1:200 TBR2 Santa Cruz Biotechnology sc-220 Rabbit polyclonal 1:350 rh ALK2 chimera Bio-techne (Wiesbaden, Germany) 637-AR Human rh 5 µM rh ALK3 chimera Bio-techne 315-BR Human rh 5 µM rh ALK6 chimera Bio-techne 505-PR Human rh 5 µM rh IgG1 chimera Bio-techne 110-HG Human rh 5 µM Open in a separate window Cat-No, Catalog-Number; rh, recombinant human.

Techniques:

Journal: Zebrafish

Article Title: Injury of Adult Zebrafish Expressing Acvr1l Q204D Does Not Result in Heterotopic Ossification

doi: 10.1089/zeb.2018.1611

Figure Lengend Snippet: rh activin A is detectable in zebrafish muscle tissue at 1 hpi. Before injection injury, adult zebrafish were anesthetized, and scales were gently removed from the right, dorsal side, just anterior to the dorsal fin (A). Zebrafish were injected at the descaled site with the indicated treatment using a microcapillary needle (B). To first test this model, adult Tg(Bre:GFP) zebrafish were injected with rh activin A (E: 5 × , H: 20 × ) or DMSO (D: 5 × , G: 20 × ), collected at 1 hpi, and analyzed for rh activin A expression by IHC. Act A was clearly detected at the Act A-injected injury site, and not in control DMSO or primary controls (C: 5 × , F: 20 × ). (F–H) Enlarged views of areas indicated by boxes in (C–E), respectively. n = 2 zebrafish per treatment group. (C–E) 5 × scale bar is 200 μm. (F–H) 20 × scale bar is 50 μm. Act A, activin A; DMSO, dimethyl sulfoxide; hpi, hours postinjury; IHC, immunohistochemistry; rh, recombinant human. Color images available online at www.liebertpub.com/zeb

Article Snippet: The rh activin A (0.1 mg/mL in dimethyl sulfoxide [DMSO], 2 μL per injection; 338-AC, R&D Systems, Minneapolis, MN) was then injected at the site of scale removal using a 100 μm diameter microcapillary needle and FemtoJet microinjection station (Eppendorf, Hamburg, Germany).

Techniques: Injection, Expressing, Immunohistochemistry, Recombinant

Journal: Zebrafish

Article Title: Injury of Adult Zebrafish Expressing Acvr1l Q204D Does Not Result in Heterotopic Ossification

doi: 10.1089/zeb.2018.1611

Figure Lengend Snippet: rh activin A injection caused tissue damage apparent at 2 dpi that resolved by 4 wpi. Adult heat-shocked Tg(Bre:GFP) and Tg(Bre:GFP);Tg(HS-acvr1l_Q204D-mCherry) zebrafish were injected with rh Activin A (B, D, F, H, J, L) or DMSO (A, C, E, G, I, K), collected at 2 dpi (A–H) and 4 wpi (I–L), paraffin sectioned, and analyzed by H&E staining (A–E, I–L) and anti-pSmad2 IHC (E–H). At 2 dpi, tissue damage was apparent, as indicated by a dashed line (A–H). In contrast, no tissue damage was apparent at 4 wpi (I–L). n = 2 zebrafish per treatment group, per time point. The 5 × scale bar is 200 μm (A–H), and the 20 × scale bar is 100 μm. (I–L). dpi, days postinjury; wpi, weeks postinjury. Color images available online at www.liebertpub.com/zeb

Article Snippet: The rh activin A (0.1 mg/mL in dimethyl sulfoxide [DMSO], 2 μL per injection; 338-AC, R&D Systems, Minneapolis, MN) was then injected at the site of scale removal using a 100 μm diameter microcapillary needle and FemtoJet microinjection station (Eppendorf, Hamburg, Germany).

Techniques: Injection, Staining